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Equine Insulin EIA 馬胰島素檢測(cè)試劑盒背景介紹：

Mercodia Equine Insulin ELISA provides a method for the quantitative determination of insulin in equine serum and plasma.

Equine Insulin EIA 馬胰島素檢測(cè)試劑盒SUMMARY AND EXPLANATION OF THE TEST

Insulin is the principal hormone responsible for the control of glucose metabolism. It issynthesised in the ?-cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in equimolar amounts into the portalcirculation. The mature insulin molecule comprises two polypeptide chains, the A chain and theB chain. The two chains are linked together by two inter-chain disulphide bridges. There is alsoan intra-chain disulphide bridge in the A chain.Secretion of insulin is mainly controlled by plasma glucose concentration, and the hormonehas a number of important metabolic actions. Its principal function is to control the uptakeand utilisation of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis arecounteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline),growth hormone and cortisol.Matching energy intake with the energy expenditure is important for the exercising horse(1-2). Parameters such as the hormones ghrelin, adiponectin and insulin have shown to play amajor role in mediating the energy balance either through their effects on feed intake or metabolic regulation. Glucose and insulin responses are modified by the diet and obesity, exerciselevel and stress also alters glucose and insulin metabolism (1-3).

Equine Insulin EIA 馬胰島素檢測(cè)試劑盒PRINCIPLE OF THE PROCEDURE

Mercodia Equine Insulin ELISA is a solid phase two-site enzyme immunoassay. It is based onthe direct sandwich technique in which two monoclonal antibodies are directed againstseparate antigenic determinants on the insulin molecule. During incubation, insulin in thesample reacts with peroxidase-conjugated anti-insulin antibodies and anti-insulin antibodiesbound to the microplate. After a simple washing step that removes unbound enzyme labelledantibody, the bound conjugate is detected by reaction with 3,3′-5,5′-tetramethylbenzidine (TMB).The reaction is stopped by the addition of acid, giving a colorimetric endpoint that can be readspectrophotometrically