亚洲欧美久久-亚洲精品国产精品国产自2022-色丁香婷婷-偷拍做爰吃奶视频免费看-中文字幕人妻丝袜二区-国产欧美日韩亚洲一二三区-国产在线观看黄色-成人欧美激情-欧美黄色a视频-隔壁老王国产在线精品-狼人伊人干-欧美日韩中文字幕-天天狠天天添日日拍-国产高清久久久-国产在线视频主播区

Rat Insulin ELISA 現貨供應背景介紹：

Mercodia Rat Insulin ELISA provides a method for the quantitative determination of insulin in rat serum or plasma.

Rat Insulin ELISA 現貨供應Summary and explanation of the test

Insulin is the principal hormone responsible for the control of glucose metabolism.It is synthesised in the ?-cells of the islets of Langerhans as the precursor,proinsulin, which is processed to form C-peptide and insulin. Both are secretedin equimolar amounts into the portal circulation. The mature insulin moleculecomprises two polypeptide chains, the A chain and the B chain. The two chainsare linked together by two inter-chain disulphide bridges. There is also an intrachain disulphide bridge in the A chain.?Secretion of insulin is mainly controlled by plasma glucose concentration,and the hormone has a number of important metabolic actions. Its principalfunction is to control the uptake and utilisation of glucose in peripheral tissuesvia the glucose transporter. This and other hypoglycaemic activities, such as theinhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by thehyperglycaemic hormones including glucagon, epinephrine (adrenaline), growthhormone and cortisol.

Rat Insulin ELISA 現貨供應

Principle of the procedureMercodia Rat Insulin ELISA is a solid phase two-site enzyme immunoassay. It isbased on the direct sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the insulin molecule. Duringincubation insulin in the sample reacts with peroxidase-conjugated anti-insulinantibodies and anti-insulin antibodies bound to micro-titer well. A simple washingstep removes unbound enzyme labeled antibody. The bound conjugate is detectedby reaction with 3,3’,5,5’-tetramethylbenzidine. The reaction is stopped by adding acid to give a colorimetric endpoint that is read spectrophotometrically。